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Section on Neural Gene Expression

Oxytocin receptor antibodies

Various western studies performed in SNGE

(Heon-Jin Lee, Geetha Kannan, Matthew Morris, and Scott Young, unpublished)

  Source MBL Everest Novus Apha Diagnostics Santa Cruz
Biotech
Santa Cruz
Biotech
SDI
  Cat. # LS-A244 EB0783 NLS244 OTR11-A C-20 H-60 Custom-made
  Antigen origin Human Human Human Rat Human Human Mouse
  Epitope location N-terminus N-terminus N-terminus C-terminal
cytoplasmic domain
C-terminus C-terminus C-terminus
  Antigen Seq. n.e. 64-74 n.e. 20 aa n.e. 330-389 332-388
  Host Rabbit Goat Rabbit Rabbit Goat Rabbit Rabbit
Western blot BOILED METHODS Liver N/S N/S N/B N/B N/S N/S N/S
Forebrain N/S N/B N/S N/B N/S N/D N/B
Olfactory Bulb N/S N/B N/S N/B N/S N/D N/B
NON BOILED METHODS Liver n.e. n.e. n.e. n.e. n.e. n.e. N/B
Forebrain n.e. n.e. n.e. n.e. n.e. n.e. N/S
Olfactory Bulb n.e. n.e. n.e. n.e. n.e. n.e. N/S
Transfected CHO cells
(Perkin-Elmer)
n.e. n.e. n.e. n.e. n.e. n.e. Specific
Epigen expression n.e. n.e. n.e. n.e. n.e. n.e. Specific
 
  ne : not examined  
  N/B : no specific band  
  N/D : specific band (50-65 kD) but no difference between WT and KO  
  N/S : non-specific bands only  

See vasopressin 1b receptor westerns.
Data as of 3/24/09

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