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Section on Neural Gene Expression

Vasopressin 1b receptor antibodies

Various western studies performed in SNGE

(Heon-Jin Lee, Geetha Kannan, Matthew Morris, & Scott Young, unpublished)

  Source Lolait lab Santa Cruz
Biotech
Life Span
Biosciences
Apha Diagnostics Santa Cruz
Biotech
SDI
  Cat. # n.e. H-90
(SC-30026)
LS-A266 AVP1B13-A D-20
(SC-18105)
Custom made
  Antigen origin Rat Human Human Rat Mouse Mouse
  Epitope location C-terminus N-terminus N-terminus N-terminus N-terminus C-terminus
  Antigen Seq. 395-412 181-270 n.e. 18 aa n.e. 339-421
  Host Rabbit Rabbit Rabbit Rabbit Goat Rabbit
Western blot BOILED METHODS perfused Liver N/B N/D N/B N/S N/B n.e.
Pituitary N/D N/D N/S N/S N/B n.e.
non-perfused Liver N/B N/D N/B N/S N/B N/S
Pituitary N/D N/D N/D N/S N/B N/S
NON BOILED METHODS perfused Liver n.e. n.e. n.e. n.e. n.e. n.e.
Pituitary n.e. n.e. n.e. n.e. n.e. n.e.
non-perfused Liver N/B n.e. n.e. n.e. n.e. N/B
Hypothalamus N/D n.e. n.e. n.e. n.e. N/D
Olfactory Bulb N/D n.e. n.e. n.e. n.e. N/B
Pituitary N/D n.e. n.e. n.e. n.e. N/D
Rat Pituitary Specific n.e. n.e. n.e. n.e. Specific
Transfected CHO cells
(Perkin-Elmer)
n.e. n.e. n.e. n.e. n.e. Specific
Epigen expression n.e. n.e. n.e. n.e. n.e. Specific
 
  ne : not examined  
  N/B : no specific band  
  N/D : specific band (50-65 kD) but no difference between WT and KO  
  N/S : non-specific bands only  

See oxytocin receptor westerns.
Data as of 3/24/09

Back to Vasopressin and Oxytocin Resources