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Transgenic Core Facility

Projects

The following table lists projects and the dates where progress has been made.

Project Updates and Schedules

Project ID Requested service Date submitted schedule
870 Mo CRISPR transgene 2016.11.07 2016.11.10 -injected and transferred 1 recipient.
867 Mo cryopreservation of sperm 2016.11.03 2016.11.30 - sperm cryo scheduled.
866 Mo cryopreservation of sperm 2016.11.03 2016.11.30 - sperm cryo scheduled.
865 Mo cryopreservation of sperm 2016.11.03 2016.11.30 - sperm cryo scheduled.
864 Mo cryopreservation of sperm 2016.11.03 2016.11.30 - sperm cryo scheduled.
863 Mo cryopreservation of sperm 2016.11.02 2016.11.10 - sperm frozen.
862 Mo cryopreservation of sperm 2016.11.02 waiting for donor
861 Mo cryopreservation of sperm 2016.11.02 waiting for donor
860 Mo ES cell targetting 2016.10.24 2016.10.28 - transferred 1 recipient. ( clone C6)
2016.11.04 - transferred 1 recipient. ( clone C6)
859 Mo rederivation of line 2016.10.27 2016.11.08 - embryos delivered.
849 Mo cryopreservation of sperm 2016.11.02 2016.11.10 - sperm frozen.
848 Mo cryopreservation of sperm 2016.11.02 waiting for donor
846 Mo CRISPR transgene 2016.10.14 2016.10.14 - transferred 3 recipients.
2016.11.03 - 4 pups.
2016.10.19 - transferred 4 recipients.
2016.11.08 - 2 pups. FD
845 Ra CRISPR transgene 2016.10.14 2016.10.14 Create floxed GnRH (?) mouse
838 Mo cryopreservation of sperm 2016.09.30 2016.10.05 - sperm frozen. QC needs to be repeated.
835 Mo CRISPR transgene 2016.09.27 2016.09.27 contact
2016.11.16 injected
2016.11.17 injected
834 Mo cryopreservation of sperm 2016.09.27 2016.10.05 - sperm cryo scheduled.
2016.10.04 - investigator didn t bring male.
2016.11.03 - contacted the investigator for the male.
833 Mo cryopreservation of sperm 2016.09.27 2016.10.05 - sperm cryo scheduled.
2016.10.04 -waiting for donor
2016.11.03 - contacted the investigator for the male.
832 Mo cryopreservation of sperm 2016.09.26 2016.09.26 - investigator needs to use the male for breeding. Will provide the male after a month.
2016.11.10 - sperm frozen.
821 Mo CRISPR transgene 2016.09.13 2016.9.15 - transferred 7 recipients. (cytoplasmic injection)
2016.10.03 - 31 pups born.
2016.09.16 - transferred 4 recipient. (pronucleus injection). no pups.
2016.10.27 - mice weaned. to be tagged.
820 Mo rederivation of line 2016.09.13 2016.10.07- transferred one recipient.
2016.10. 27 - 14 pups born.
2016.10.19 - transferred 1 recipient. no pups.
819 Mo rederivation of line 2016.09.13 2016.10.07 - transferred one recipient. no pups.
2016.11.04 - transferred 1 recipient (transferred only 5 two cell stage embryos)
2016.11.08 - transferred 3 recipients.
803 Mo rederivation of line 2016.08.12 2016.08.31 - received the frozen sperm ( 1 vial )
2016.09.16 - transferred 3 recipients.
2016.10.05 - 32 pups.
2016.10.19 - transferred 2 recipients.
2016.11.07 - 10 pups.
2016.11.02 - serology request submitted. ( 32 pups)
796 Ra CRISPR transgene 2016.08.01 2016.08.03 - transferred 2 recipients.
2016.08.17 - euthanized the recipients and harvested the samples.
2016.08.22 - 31 samples sent to the investigator for genotyping.
2016.08.28 - 40% positive for the mutation.
2016.08.24 - transferred 3 recipients.
2016.09.15 - 37 pups born. mice weaned.
2016.09.02 - transferred 1 recipient. ( injected donor plasmid )
2016.09.24 - 12 pups.
2016.09.09 - transferred 2 recipients. (injected donor plasmid)
2016.10.01 - 28 pups born.
2016.09.29 - transferred 1 recipient. (injected donor plasmid)
2016.10.21 - 11 pups born
2016.11.02 - tail biopsies sent to the investigator ( 41)
2016.11.15 - ASP not ready to transfer the positive rats. mating to be set up for the positive rats.
782 Mo cryopreservation of sperm 2016.06.21 2016.06.29 - sperm frozen.
2016.07.08 - QC - 22%
742 Ra rederivation of line 2016.04.12 2016.04.06 - 2 cell embryos harvested. no recipient. Froze the embryos.
2016.05.24 - didn t get any embryos.
2016.06.07 - didn t get any recipients.
2016.06.09 - transferred 2 recipients. no pups.
2016.06.15 - transferred 1 recipient.
2016.06.30 - transferred 1 recipient. Looks pregnant.
2016.07.07 - 20 pups.
2016.07.23 - 19 pups.
2016.07.29 - serology request submitted.
2016.08.18 - rats released to B1D19 and tagged.
688 Mo rederivation of line 2015.12.30 2016.01.08 - transferred 2 recipients. (one for I69 & one for I24 ML4)
2016.01.27 - I24 ML4 - 7 pups.
2016.01.28 - I69 - 4 pups.
2016.02.19- mice weaned .
2016.02.26 - mice tagged and tailed.
2016.03.25 - breeding set up for the positive male.
2016.06.06 - have G1 mice. ready to ship the founders.
67 Ra CRISPR transgene 2015.12.21 2016.03.16 - injected RNA
2016.04.06 - 6 pups negative
2016.04.12 - tail biopsies sent for genotyping.
2016.04.22 - transferred 2 recipients. no pups.
2016.05.13 - transferred 1 recipient.
2016.06.06 - 2 pups. weaned and tagged.
2016.05.26 - transferred 1 recipient. no pups.
2016.06.28 - 2 pups weaned and ear tagged.
2016.06.30 - transferred 2 recipients.
2016.07.22 - 11 pups. weaned and tagged.
2016.07.08 - transferred 1 recipient. no pups.
2016.07.15 - transferred 2 recipients.
2016.08.07 - 7 pups. weaned and tagged.
2016.07.22 - transferred 1 recipient.
2016.08.15 - 3 pups.
2016.08.31 - tail biopsies (20) given to Nick Ryba.
2016.09.02 - more than half targeted and 4 are homozygous.



53 Mo CRISPR transgene 2015.10.19 2015.10.27 - transferred 2 recipients.
2015.11.15 - 23 pups.
2015.11.18 - transferred 3 recipients.
2015.12.07 - 37 pups.
2015.12.28 - the samples from the first batch of litter sent to the investigator.
2016.01.04 - samples from the remaining litter sent to the investigator.
34 Mo ES cell targetting 2015.06.18 2015.09.15 - received ES cells.
2015.09.25 - transferred 2 recipients.
2015.10.15 - 5 pups. 2 good chimeras. 1male and 1 female.
2015.09.29 - transferred 1 recipient.
2015.10.01 - transferred 1 recipient.
2015.10.21 - 7 pups. 3 died. no chimeras.
2015.11.04 - transferred 1 recipient.
2015.11.24 - 6 pups. 2 died. 2 are agouti. 1 male and 1 female.
2015.12.17 - transferred 2 recipients.
2016.01.05 - 14 pups. 2 male agouti.
2016.02.29 - tail biopsies ( Htr7 F1) picked up by the investigator. none of them are transgenic.
2016.04.13 - more F1 mice (6) ear tagged. one pup agouti.
2016.04.19 - tail biopsies picked up by the investigator.
2016.05.02 - genotyping is done in the lab.
2016.06.07 - more tail biopsies given to the investigator. all are WT.
2016.10.03 - more tail biopsies picked up by the investigator.
all are WT.
2016.11.01 - transferred 2 recipients.
2016.11.10 - transferred 2 recipients.
33 Mo ES cell targetting 2015.06.18 2015.11.17- ES cells arrive
2015.11.24 - A02 - transferred 1 recipient. no pups.
2015.12.03 - A02 - transferred 1 recipient. no pups.
2016.01.12 - D04 - transferred 1 recipient.
2016.01.31 - 9 pups. no chimeras.
2016.01.21 - D04 - transferred 1 recipient.
2016.02.09 - 2 pups. 1 female pup agouti ( chimera % - <30)
2016.03.03 - transferred 1 recipient. no pups.
2016.03.10 - transferred 1 recipient.
2016.03.30 - 4 pups. no chimeras.
2016.03.16 - transferred 1 recipient. 1 pup. FD.
2016.06.03 - transferred 1 recipient clone D04.
2016.06.22 - 4 pups born. 2 male are chimeras. ( chimera % - 20- 30% )
2016.08.26 - breeding setup for the founders.
2016.10.15 - 3 litters born. no GT.