Immunohistochemical detection of OT neurophysin or processed OT

Immunohistochemical detection of OT neurophysin (A,B) or of processed OT (C-F) in the PVN (A-D) or pituitary (E-F) of wild-type (A, C, E) and homozygous mutant (B, D, F) mice. There are numerous heavily stained neurons and axon fibers (arrows) present in the wild-type mice stained with either antibody. No staining was observed with either the neurophysin or the OT antibody in the mutant mouse. OT immunoreactivity was abundant in the posterior pituitary (p) of the WT mouse, but absent from that of the HO mouse. 3 is the third ventricle, a is anterior pituitary, i is intermediate lobe. Bar equals 1mm.